In:
Stroke, Ovid Technologies (Wolters Kluwer Health), Vol. 55, No. Suppl_1 ( 2024-02)
Abstract:
Background: Acute brain injuries, including aneurysmal subarachnoid hemorrhage (aSAH), ischemic stroke (IS), and traumatic brain injury (TBI), entail complex recovery processes involving brain plasticity and synaptic regeneration mechanisms. Exploring lipidomic profiles, microRNA expression, and metabolomic changes can yield insights into recovery mechanisms, unveil conserved molecular associations, and identify potential biomarkers. Hypotheses: We hypothesized that regardless of injury type, variations in lipidomic and metabolomic profiles, along with differentially expressed microRNAs, share common characteristics. These changes likely mirror essential biological mechanisms and may correlate with outcomes. Methods: We studied a prospective cohort of patients with IS, aSAH, and TBI (N=71). Serum samples were collected once, followed by comprehensive profiling: lipidomics (Lipidyzer™ Platform), metabolomics (Orbitrap Profiling), and microRNA sequencing (DESeq2). Results: Univariate analysis unveiled candidate molecules predictive of outcomes. Multivariate combinatory linear discriminant analysis (LDA) demonstrated significant prognostic potential for favorable outcomes. The LDA equation combining eight biomarkers was: -0.239[LPC17:0] - 1.125[TAG51:2:FA15:0] + [TAG51:2:FA18:1] + [D-glucuronic Acid] + 0.416[hsa-miR-146b-3p] + 0.082[hsa-miR-485-3p] + 0.433[hsa-miR-5010-5p] + 0.727[hsa-miR-485-5p] , effectively predicting favorable outcomes. The AUC was 95.8%, 95% CI (0.88 - 1.00), p-value 0.023, indicating strong discriminatory power of the combinatory biomarker (OR 8.7, 95% CI 1.35-56.9). Conclusion: Findings reveal a shared pattern of lipidomic, metabolomic, and microRNA changes across distinct acute brain injuries. This warrants further investigation and validation, offering prospects for biomarker development, mechanistic validation, and therapeutic interventions in brain recovery. These shared features underscore the importance of interdisciplinary research for targeted, personalized interventions in diverse acute brain injuries.
Type of Medium:
Online Resource
ISSN:
0039-2499
,
1524-4628
DOI:
10.1161/str.55.suppl_1.TMP25
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2024
detail.hit.zdb_id:
1467823-8
