In:
Angiology, SAGE Publications, Vol. 38, No. 1 ( 1987-01), p. 51-55
Abstract:
In this study, TMB-8, an intracellular calcium antagonist, and verapamil, an extracellular calcium antagonist, were used simultaneously to elucidate the role of calcium in the pulmonary vasopressor response induced by PGF 2α and U46619. The pulmonary vasoconstrictor action of these two agonists was evaluated in the canine isolated lung lobe preparation. Lobar arterial pressure was constantly monitored and changes in arterial pressure were recorded as a percentage from baseline. Control responses to PGF 2α (42.0±8.2%) and U46619 (47.2±7.0%) were obtained prior to the administration of TMB-8 and verapamil. After administration of TMB-8 and verapamil, the PGF 2α (7.4±3.1%) and U46619 (28.8±6.2%) responses were significantly attenutated. We conclude that the PGF 2α pressor response is dependent on a TMB-8-sensitive intracellular calcium pool and a verapamil-sensitive slow-channel calcium influx. In contrast, the degree of attenuation of the U46619 response was similar to the vasopressor response in the presence of verapamil alone, as described previously. This indicates a direct dependence on extracellular calcium. An additional source of calcium insensitive to verapamil and TMB-8 may also be activated and contribute to the pulmonary vasoconstrictor action. These results suggest that each agonist possesses a mechanism of action distinctly different from the other.
Type of Medium:
Online Resource
ISSN:
0003-3197
,
1940-1574
DOI:
10.1177/000331978703800107
Language:
English
Publisher:
SAGE Publications
Publication Date:
1987
detail.hit.zdb_id:
2065911-8
