In:
Angiology, SAGE Publications, Vol. 39, No. 3 ( 1988-03), p. 246-252
Abstract:
In earlier investigations it has been found that high arterial blood pressure of salt-sensitive DAHL rats (DAHL-S) that received a diet con taining 8% NaCl could be normalized by chronic treatment with specific calcium antagonists, as for instance, nitrendipine or anipamil. Parallel measurements on heart and arterial vasculature with atomic absorption spectrometry revealed that there was a rise in absolute calcium content upon development of hypertension, whereas under the influence of ni trendipine, not only the blood pres sure and heart weight but also myocardial (and arterial) tissue cal cium content remained in the physio logic range. In the present study, an additional determination of the free intracellu lar calcium ion concentration with Ca-sensitive microelectrodes was car ried out. For technical reasons, the measurements were restricted to iso lated resting rat papillary muscles (stretched to optimal length in a per fusion bath of 1.5 ml at 30°C in nor mal Tyrode solution). The impale ment of the electrodes was considered adequate when the heights of Ca po tential and membrane potential were constant for more than twenty min utes. In order to produce severe sys temic hypertension, a group of six-week-old DAHL-S rats was fed with an 8% NaCl diet over five weeks. Another group of DAHL-S rats received additionally 150 mg/kg nitrendipine twice a day orally. Salt- resistant DAHL-R rats, also fed with an 8% NaCl diet, served as controls. The findings of this preliminary study are as follows (n = 5 in each group):
Type of Medium:
Online Resource
ISSN:
0003-3197
,
1940-1574
DOI:
10.1177/000331978803900307
Language:
English
Publisher:
SAGE Publications
Publication Date:
1988
detail.hit.zdb_id:
2065911-8
