In:
Journal of Histochemistry & Cytochemistry, SAGE Publications, Vol. 50, No. 3 ( 2002-03), p. 289-303
Abstract:
Intracellular signaling relies on the orchestrated cooperation of signaling proteins and modules, their intracellular localization, and membrane trafficking. Recently, a repertoire of fluorescence-based techniques, which significantly increases our potential for detailed studies of the involved mechanisms, has been introduced. Microscopic techniques with increased resolution have been combined with improved techniques for detection of signaling proteins. Transfections of fluorescently tagged proteins have allowed in vivo microscopy of their trafficking and interactions with other proteins and intracellular structures. We present an overview of general signaling principles and a description of techniques based on fluorescent microscopy suited for studies of signaling mechanisms.
Type of Medium:
Online Resource
ISSN:
0022-1554
,
1551-5044
DOI:
10.1177/002215540205000301
Language:
English
Publisher:
SAGE Publications
Publication Date:
2002
detail.hit.zdb_id:
1421306-0
SSG:
12