In:
Alternatives to Laboratory Animals, SAGE Publications, Vol. 20, No. 2 ( 1992-04), p. 258-261
Kurzfassung:
Isolated human platelets represent a model system which could be used in in vitro cell toxicology for mechanistic studies on the short-term effects of cytotoxic compounds. We have recorded changes in the concentration of free cytosolic Ca 2+ ([Ca 2+ ] i ) with Fura 2 in isolated human platelets treated with sphingosine, a compound suggested to be a specific inhibitor of protein kinase C (PKC). We huve shown that sphingosine is able to induce a rapid and transient release of Ca 2+ from the intracellular stores in platelets. Furthermore, a massive efflux of released Ca 2+ was shown. These effects of sphingosine on the human platelet might leave the cell incapable of performing Ca 2+ -dependent reactions, including PKC-mediated phosphorylation, upon stimulation subsequent to sphingosine-treatment.
Materialart:
Online-Ressource
ISSN:
0261-1929
,
2632-3559
DOI:
10.1177/026119299202000214
Sprache:
Englisch
Verlag:
SAGE Publications
Publikationsdatum:
1992
ZDB Id:
2390905-5
SSG:
12,22
