In:
Toxicology and Industrial Health, SAGE Publications, Vol. 32, No. 6 ( 2016-06), p. 1042-1051
Kurzfassung:
This study investigated the effects of extremely low frequency electromagnetic fields (ELF-EMFs) on human fetal scleral fibroblasts (HFSFs). HFSFs were subjected to 50 Hz artificial ELF-EMFs generated by Helmholtz coils with 0.1, 0.2, 0.5, and 1.0 mT field intensities for 6 to 48 h. The viability and factors involved in scleral structuring of HFSFs were determined. The growth rate of HFSFs significantly decreased after only 24 h of exposure to ELF-EMFs (0.2 mT). The messenger RNA (mRNA) expression of collagen type I (COL1A1) decreased and expression of matrix metalloproteinase-2 (MMP-2) increased significantly. There was a decrease in tissue inhibitor of MMP-2 mRNA levels between treated and control cells only at the 1.0 mT intensity level. Transforming growth factor beta-2 mRNA increased in exposed cells, and, simultaneously, fibroblast growth factor-2 mRNA levels decreased. The protein expressions of COL1A1 and MMP-2 were also significantly altered subsequent to exposure ( p 〈 0.05). This study shows that ELF-EMFs had biological effects on HFSFs and could cause abnormality in scleral collagen.
Materialart:
Online-Ressource
ISSN:
0748-2337
,
1477-0393
DOI:
10.1177/0748233714545837
Sprache:
Englisch
Verlag:
SAGE Publications
Publikationsdatum:
2016
ZDB Id:
2010891-6
SSG:
12
