In:
Cancer Control, SAGE Publications, Vol. 28 ( 2021-01-01), p. 107327482110271-
Abstract:
Circulating tumor cells (CTCs) with an epithelial-mesenchymal transition phenotype in peripheral blood may be a useful marker of carcinomas with poor prognosis. The aim of this study was to determine the prognostic significance of CTCs expressing Krüppel-like factor 8 (KLF8) and vimentin in pancreatic cancer (PC). Methods: CTCs were isolated by immunomagnetic separation from the peripheral blood of 40 PC patients before undergoing surgical resection. Immunocytochemistry was performed to identify KLF8 + and vimentin + CTCs. The associations between CTCs and time to recurrence (TTR), clinicopathologic factors, and survival were assessed. Univariate and multivariate analyzes were performed to identify risk factors. Results: Patients with CTCs ( n = 30) had a higher relapse rate compared to those without ( n = 10) (70.0% vs 20.0%; P 〈 0.01). The proportion of KLF8 + /vimentin + CTCs to total CTCs was inversely related to TTR ( r = −0.646; P 〈 0.01); TTR was reduced in patients with 〉 50% of CTCs identified as KLF8 + /vimentin + ( P 〈 0.01). Independent risk factors for recurrence were perineural invasion and 〉 50% KLF8 + /vimentin + CTCs (both P 〈 0.05). Conclusion: Poor prognosis can be predicted in PC patients when 〉 50% of CTCs are positive for KLF8 and vimentin.
Type of Medium:
Online Resource
ISSN:
1073-2748
,
1073-2748
DOI:
10.1177/10732748211027163
Language:
English
Publisher:
SAGE Publications
Publication Date:
2021
detail.hit.zdb_id:
2004182-2
