In:
Journal of Dental Research, SAGE Publications, Vol. 87, No. 12 ( 2008-12), p. 1172-1176
Abstract:
Information concerning phenotypic heterogeneity of Streptococcus mutans in carious dentin is sparse. Matrix-assisted laser-desorption/ionization-time-of-flight mass-spectrometry (MALDI-TOF-MS) facilitates the phenotypic differentiation of bacteria to the subspecies level. To verify a supposed influence of restorative treatment on the phenotypic heterogeneity of S. mutans, we isolated and compared a total of 222 S. mutans strains from dentin samples of 21 human deciduous molars during caries excavation (T 1 ) and 8 wks (T 2 ) after removal of the temporary restoration. Phenotypic heterogeneity was determined by MALDI-TOF-MS and hierarchical clustering. Thirty-six distinct S. mutans phenotypes could be identified. Although indistinguishable phenotypes were found in the same teeth at T 1 and T 2 , as well as in different teeth of individual participants, the phenotypic heterogeneity increased significantly, from 1.4 phenotypes per S. mutans-positive dentin sample at T 1 to 2.2 phenotypes at T 2 . We attribute this to an adaptation of S. mutans to the modified environment under the restoration following caries excavation.
Type of Medium:
Online Resource
ISSN:
0022-0345
,
1544-0591
DOI:
10.1177/154405910808701203
Language:
English
Publisher:
SAGE Publications
Publication Date:
2008
detail.hit.zdb_id:
2057074-0
