In:
Blood, American Society of Hematology, Vol. 40, No. 6 ( 1972-12-01), p. 823-835
Abstract:
Tryptic peptides characteristic of mouse hemoglobin α- and diffuse-type β-chains were isolated from dimethylsulfoxide-treated mouse leukemia virus-infected cell cultures. Quantitative estimates of in vitro hemoglobin synthesis by such cultures were obtained by two different methods: by analysis of radioactive amino acid incorporation, and by single-cell radial immunodiffusion assay. The amounts produced, approximately 4 pg/cell/17 hr, reflect a synthetic capacity that is probably sufficient for 32P labeling of hemoglobin messenger RNAs (mRNA) in quantities commensurate with structural characterization of mRNAs by radioautographic methods.
Type of Medium:
Online Resource
ISSN:
0006-4971
,
1528-0020
DOI:
10.1182/blood.V40.6.823.823
Language:
English
Publisher:
American Society of Hematology
Publication Date:
1972
detail.hit.zdb_id:
1468538-3
detail.hit.zdb_id:
80069-7