In:
European Respiratory Journal, European Respiratory Society (ERS), Vol. 48, No. 2 ( 2016-08), p. 459-469
Abstract:
Circulating mononuclear cells may play an important role for the vascular remodelling in pulmonary arterial hypertension (PAH), but studies addressing multiple progenitor populations are rare and inconsistent. We used a comprehensive fluorescence-activated cell sorting analysis of circulating mononuclear cells in 20 PAH patients and 20 age- and sex-matched controls, and additionally analysed CD133 + cells in the lung tissue of five PAH transplant recipients and five healthy controls (donor lungs). PAH patients were characterised by increased numbers of circulating CD133 + cells and lymphopenia as compared with control. In PAH, CD133 + subpopulations positive for CD117 or CD45 were significantly increased, whereas CD133 + CD309 + , CD133 + CXCR2 + and CD133 + CD31 + cells were decreased. In CD133 + cells, SOX2, Nanog, Ki67 and CXCR4 were not detected, but Oct3/4 mRNA was present in both PAH and controls. In the lung tissue, CD133 + cells included three main populations: type 2 pneumocytes, monocytes and undifferentiated cells without significant differences between PAH and controls. In conclusion, circulating CD133 + progenitor cells are elevated in PAH and consist of phenotypically different subpopulations that may be up- or downregulated. This may explain the inconsistent results in the literature. CD133 + type 2 pneumocytes in the lung tissue are not associated with circulating CD133 + mononuclear cells.
Type of Medium:
Online Resource
ISSN:
0903-1936
,
1399-3003
DOI:
10.1183/13993003.01523-2015
Language:
English
Publisher:
European Respiratory Society (ERS)
Publication Date:
2016
detail.hit.zdb_id:
2834928-3
detail.hit.zdb_id:
1499101-9
