In:
BMC Genomics, Springer Science and Business Media LLC, Vol. 14, No. 1 ( 2013-12)
Kurzfassung:
Mycobacterium tuberculosis Beijing strains are characterized by a large number of IS 6110 copies, suggesting the potential implication of this element in the virulence and capacity for rapid dissemination characteristic of this family. This work studies the insetion points of IS 6110 in high-copy clinical isolates specifically focusing on the Beijing genotype. Results In the present work we mapped the insertion points of IS 6110 in all the Beijing strains available in the literature and in the DNA sequence databases. We generated a representative primer collection of the IS 6110 locations, which was used to analyse 61 high-copy clinical isolates. A total of 440 points of insertion were identified and analysis of their flanking regions determined the exact location, the direct repeats (DRs), the orientation and the distance to neighboring genes of each copy of IS 6110 . We identified specific points of insertion in Beijing strains that enabled us to obtain a dendrogram that groups the Beijing genotype. Conclusions This work presents a detailed analysis of locations of IS 6110 in high-copy clinical isolates, showing points of insertion present with high frequency in the Beijing family and absent in other strains.
Materialart:
Online-Ressource
ISSN:
1471-2164
DOI:
10.1186/1471-2164-14-422
Sprache:
Englisch
Verlag:
Springer Science and Business Media LLC
Publikationsdatum:
2013
ZDB Id:
2041499-7
SSG:
12
