In:
BMC Immunology, Springer Science and Business Media LLC, Vol. 12, No. 1 ( 2011-12)
Abstract:
We previously showed that co-immunization with a protein antigen and a DNA vaccine coding for the same antigen induces CD40 low IL-10 high tolerogenic DCs, which in turn stimulates the expansion of antigen-specific CD4 + CD25 - Foxp3 + regulatory T cells (CD25 - iTreg). However, it was unclear how to choose the antigen sequence to maximize tolerogenic antigen presentation and, consequently, CD25 - iTreg induction. Results In the present study, we demonstrated the requirement of highly antigenic epitopes for CD25 - iTreg induction. Firstly, we showed that the induction of CD25 - iTreg by tolerogenic DC can be blocked by anti-MHC-II antibody. Next, both the number and the suppressive activity of CD25 - iTreg correlated positively with the overt antigenicity of an epitope to activate T cells. Finally, in a mouse model of dermatitis, highly antigenic epitopes derived from a flea allergen not only induced more CD25 - iTreg, but also more effectively prevented allergenic reaction to the allergen than did weakly antigenic epitopes. Conclusions Our data thus indicate that efficient induction of CD25 - iTreg requires highly antigenic peptide epitopes. This finding suggests that highly antigenic epitopes should be used for efficient induction of CD25 - iTreg for clinical applications such as flea allergic dermatitis.
Type of Medium:
Online Resource
ISSN:
1471-2172
DOI:
10.1186/1471-2172-12-27
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2011
detail.hit.zdb_id:
2041500-X
