In:
Proteome Science, Springer Science and Business Media LLC, Vol. 9, No. 1 ( 2011-12)
Abstract:
Phytophthora sojae causes soybean root and stem rot, resulting in an annual loss of 1-2 billion US dollars in soybean production worldwide. A proteomic technique was used to determine the effects on soybean hypocotyls of infection with P. sojae . Results In the present study, 46 differentially expressed proteins were identified in soybean hypocotyls infected with P. sojae , using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization tandem time of flight (MALDI-TOF/TOF). The expression levels of 26 proteins were significantly affected at various time points in the tolerant soybean line, Yudou25, (12 up-regulated and 14 down-regulated). In contrast, in the sensitive soybean line, NG6255, only 20 proteins were significantly affected (11 up-regulated and 9 down-regulated). Among these proteins, 26% were related to energy regulation, 15% to protein destination and storage, 11% to defense against disease, 11% to metabolism, 9% to protein synthesis, 4% to secondary metabolism, and 24% were of unknown function. Conclusion Our study provides important information on the use of proteomic methods for studying protein regulation during plant-oomycete interactions.
Type of Medium:
Online Resource
ISSN:
1477-5956
DOI:
10.1186/1477-5956-9-52
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2011
detail.hit.zdb_id:
2141087-2
