In:
Journal of Hematology & Oncology, Springer Science and Business Media LLC, Vol. 3, No. 1 ( 2010-12)
Abstract:
In a human T-cell acute lymphoblastic leukemia (T-ALL) cell line (Molt-4), siRNA-mediated suppression of BCL11B expression was shown to inhibit proliferation and induce apoptosis, functions which may be related to genes involved in apoptosis (such as TNFSF10 and BCL2L1 ) and TGF-β pathways (such as SPP1 and CREBBP ). Methods The expression levels of the above mentioned genes and their correlation with the BCL11B gene were analyzed in patients with T-ALL using the TaqMan and SYBR Green I real-time polymerase chain reaction technique. Results Expression levels of BCL11B, BCL2L1 , and CREBBP mRNA in T-ALL patients were significantly higher than those from healthy controls ( P 〈 0.05). In T-ALL patients, the BCL11B expression level was negatively correlated with the BCL2L1 expression level ( r s = -0.700; P 〈 0.05), and positively correlated with the SPP1 expression level ( r s = 0.683; P 〈 0.05). In healthy controls, the BCL11B expression level did not correlate with the TNFSF10 , BCL2L1 , SPP1 , or CREBBP expression levels. Conclusions Over-expression of BCL11B might play a role in anti-apoptosis in T-ALL cells through up-regulation of its downstream genes BCL2L1 and CREBBP .
Type of Medium:
Online Resource
ISSN:
1756-8722
DOI:
10.1186/1756-8722-3-44
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2010
detail.hit.zdb_id:
2429631-4
