In:
Journal of Experimental & Clinical Cancer Research, Springer Science and Business Media LLC, Vol. 29, No. 1 ( 2010-12)
Kurzfassung:
This study aimed to determine the miRNA profile in breast cancer stem cells (BCSCs) and to explore the functions of characteristic BCSC miRNAs. Methods We isolated ESA + CD44 + CD24 -/low BCSCs from MCF-7 cells using fluorescence-activated cell sorting (FACS). A human breast cancer xenograft assay was performed to validate the stem cell properties of the isolated cells, and microarray analysis was performed to screen for BCSC-related miRNAs. These BCSC-related miRNAs were selected for bioinformatic analysis and target prediction using online software programs. Results The ESA + CD44 + CD24 -/low cells had up to 100- to 1000-fold greater tumor-initiating capability than the MCF-7 cells. Tumors initiated from the ESA + CD44 + CD24 -/low cells were included of luminal epithelial and myoepithelial cells, indicating stem cell properties. We also obtained miRNA profiles of ESA + CD44 + CD24 -/low BCSCs. Most of the possible targets of potential tumorigenesis-related miRNAs were oncogenes, anti-oncogenes or regulatory genes. Conclusions We identified a subset of miRNAs that were differentially expressed in BCSCs, providing a starting point to explore the functions of these miRNAs. Evaluating characteristic BCSC miRNAs represents a new method for studying breast cancer-initiating cells and developing therapeutic strategies aimed at eradicating the tumorigenic subpopulation of cells in breast cancer.
Materialart:
Online-Ressource
ISSN:
1756-9966
DOI:
10.1186/1756-9966-29-174
Sprache:
Englisch
Verlag:
Springer Science and Business Media LLC
Publikationsdatum:
2010
ZDB Id:
2430698-8
