In:
Rice, Springer Science and Business Media LLC, Vol. 14, No. 1 ( 2021-12)
Abstract:
Rice blast caused by Magnaporthe oryzae is one of the most destructive diseases of rice. An increasing number of microRNAs (miRNAs) have been reported to fine-tune rice immunity against M. oryzae and coordinate with growth and development. Results Here, we showed that rice microRNA159a ( Osa-miR159a ) played a positive role in rice resistance to M. oryzae . The expression of Osa-miR159a was suppressed in a susceptible accession at 12, 24, and 48 h post-inoculation (hpi); it was upregulated in a resistant accession of M. oryzae at 24 hpi. The transgenic rice lines overexpressing Osa-miR159a were highly resistant to M. oryzae . In contrast, the transgenic lines expressing a short tandem target mimic (STTM) to block Osa-miR159a showed enhanced susceptibility. Knockout mutations of the target genes of Osa-miR159a , including OsGAMYB , OsGAMYBL , and OsZF , led to resistance to M. oryzae . Alteration of the expression of Osa-miR159a impacted yield traits including pollen and grain development. Conclusions Our results indicated that Osa-miR159a positively regulated rice immunity against M. oryzae by downregulating its target genes. Proper expression of Osa-miR159a was critical for coordinating rice blast resistance with grain development.
Type of Medium:
Online Resource
ISSN:
1939-8425
,
1939-8433
DOI:
10.1186/s12284-021-00469-w
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2021
detail.hit.zdb_id:
2457103-9