In:
BMC Plant Biology, Springer Science and Business Media LLC, Vol. 21, No. 1 ( 2021-12)
Abstract:
Trichomes are excellent model systems for the analysis of cell differentiation and play essential roles in plant protection. From cucumber inbred line ‘WD1’, we identified an EMS-induced trichome abnormally developing mutant, nps , which exhibited smaller, denser and no pyramid-shaped head trichomes. Results Using F 2 and BC 1 populations constructed from a cross between nps and ‘9930’, the genetic analysis showed that the nps trait is controlled by a single recessive nuclear gene. We identified CsNps by map-based cloning with 576 individuals of the F 2 population generated from the cross of nps and inbred line ‘9930’. The CsNps was located at a 13.4-kb genomic region on chromosome 3, which region contains three predicted genes. Sequence analysis showed that only one single nucleotide mutation (C → T) between 9930 and nps was found in the second exon of Csa3G748220 , a plant-specific class I HD-Zip gene. The result of allelism test also indicated that nps is a novel allelic mutant of Mict ( Micro-trichome ). Thus, nps was renamed mict-L130F . By comparing the transcriptome of mict-L130F vs WD1 and 06–2 ( mict ) vs 06–1 (wildtype, near-isogenic line of 06–2), several potential target genes that may be related to trichome development were identified. Conclusions Our results demonstrate that Mict-L130F is involved in the morphogenesis of trichomes. Map-based cloning of the Mict-L130F gene could promote the study of trichome development in cucumber.
Type of Medium:
Online Resource
ISSN:
1471-2229
DOI:
10.1186/s12870-021-02955-1
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2021
detail.hit.zdb_id:
2059868-3
SSG:
12
