In:
BMC Biology, Springer Science and Business Media LLC, Vol. 17, No. 1 ( 2019-12)
Kurzfassung:
Optogenetic silencing techniques have expanded the causal understanding of the functions of diverse neuronal cell types in both the healthy and diseased brain. A widely used inhibitory optogenetic actuator is e Np HR3.0, an improved version of the light-driven chloride pump halorhodopsin derived from Natronomonas pharaonis . A major drawback of e Np HR3.0 is related to its pronounced inactivation on a time-scale of seconds, which renders it unsuited for applications that require long-lasting silencing. Results Using transgenic mice and Xenopus laevis oocytes expressing an e Np HR3.0-EYFP fusion protein, we here report optimized photo-stimulation techniques that profoundly increase the stability of e Np HR3.0-mediated currents during long-term photo-stimulation. We demonstrate that optimized photo-stimulation enables prolonged hyperpolarization and suppression of action potential discharge on a time-scale of minutes. Conclusions Collectively, our findings extend the utility of e Np HR3.0 to the long-lasting inhibition of excitable cells, thus facilitating the optogenetic dissection of neural circuits.
Materialart:
Online-Ressource
ISSN:
1741-7007
DOI:
10.1186/s12915-019-0717-6
Sprache:
Englisch
Verlag:
Springer Science and Business Media LLC
Publikationsdatum:
2019
ZDB Id:
2133020-7
SSG:
12