In:
Orphanet Journal of Rare Diseases, Springer Science and Business Media LLC, Vol. 16, No. 1 ( 2021-12)
Abstract:
Bleeding anomalies have been reported in patients affected by Noonan syndrome. No study has been performed in patients with molecularly confirmed RASopathy. We aimed to characterize the frequency and types of bleeding disorders in patients with RASopathies and evaluate any significant association with laboratory findings. Patients and methods Forty-nine individuals ( PTPN11 , n = 27; SOS1 , n = 7; RIT1 , n = 3; SPRED1 , n = 1; LZTR1 , N = 3; RAF1 , n = 2; BRAF , n = 4; MEK1 , n = 1; MEK2 , n = 1), and 49 age- and sex-matched controls were enrolled. The “Paediatric Bleeding Questionnaire Scoring Key” was administered to patients and families. Laboratory screening tests including clotting factors dosing, platelet count, Prothrombin Time and Partial Thromboplastin Time, were employed both in patients and controls to characterize the bleeding diathesis. A subgroup of 29/49 patients and 29/49 controls was also tested for platelet function. Results Regardless of the gene involved, pathological paediatric bleeding scores were recorded in 14/49 (28.5%) patients. Indeed, 7 were mutated in PTPN11 , 3 in SOS1 , 2 in RIT1 , 1 in BRAF , and 1 in MEK1 . Compared to patients with normal bleeding scores, those with pathologic bleeding score showed higher prevalence of splenomegaly ( p = 0.006), prolonged aPTT ( p = 0.04), lower levels of coagulation factor V (FV, p = 0.001), FVII ( p = 0.003), FX ( p = 0.0008) and FXIII ( p = 0.002), higher vWAg ( p = 0.04), and lower platelet sensitivity to Ristocetin ( p = 0.001), arachidonic acid (AA) ( p = 0.009) and collagen ( p = 0.01). The presence of hematomas inversely correlated with factor V ( p = 0.002), factor VII ( p = 0.003), factor X ( p = 0.002) and factor XIII ( p = 0.004) levels, and directly correlated with platelet response to collagen ( p = 0.02) and AA ( p = 0.01). The presence of splenomegaly directly correlated with the presence of hematoma ( p = 0.006), platelet response to Ristocetin ( p = 0.04) and AA ( p = 0.04), and inversely correlated with factor V levels ( p = 0.03). Conclusions Patients with RASopathies and a bleeding tendency exhibit multiple laboratory abnormalities, including platelet-related disorders. Splenomegaly is frequently detected and might be a suggestive sign for qualitative platelet dysfunction. A comprehensive clinical assessment should be carried out at diagnosis, during the follow-up and before any surgical procedures. Since there is currently no consensus on management of bleeding complications, it is important that physicians closely monitor these patients.
Type of Medium:
Online Resource
ISSN:
1750-1172
DOI:
10.1186/s13023-021-02122-7
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2021
detail.hit.zdb_id:
2225857-7
