In:
Biotechnology for Biofuels, Springer Science and Business Media LLC, Vol. 14, No. 1 ( 2021-12)
Kurzfassung:
Wax esters (WE) are neutral lipids that consist of a fatty alcohol esterified to a fatty acid. WE are valuable feedstocks in industry for producing lubricants, coatings, and cosmetics. They can be produced chemically from fossil fuel or plant-derived triacylglycerol. As fossil fuel resources are finite, the synthesis of WE in transgenic plants may serve as an alternative source. As chain length and desaturation of the alcohol and acyl moieties determine the physicochemical properties of WE and their field of application, tightly controlled and tailor-made WE synthesis in plants would be a sustainable, beneficial, and valuable commodity. Here, we report the expression of ten combinations of WE producing transgenes in Arabidopsis thaliana . In order to study their suitability for WE production in planta , we analyzed WE amount and composition in the transgenic plants. Results The transgenes consisted of different combinations of a FATTY ACYL-COA/ACP REDUCTASE ( FAR ) and two WAX SYNTHASES/ACYL-COA:DIACYLGLYCEROL O -ACYLTRANSFERASES ( WSD ), namely WSD2 and WSD5 from the bacterium Marinobacter aquaeoleoi . We generated constructs with and without plastidial transit peptides to access distinct alcohol and acyl substrate pools within A. thaliana cells. We observed WE formation with plastid and cytosol-localized FAR and WSD in seeds. A comparative WE analysis revealed the production of shorter and more saturated WE by plastid-localized WE biosynthesis compared to cytosolic WE synthesis. Conclusions A shift of WE formation into seed plastids is a suitable approach for tailor-made WE production and can be used to synthesize WE that are mainly derived from mid- and long-chain saturated and monounsaturated substrates.
Materialart:
Online-Ressource
ISSN:
1754-6834
DOI:
10.1186/s13068-021-02062-1
Sprache:
Englisch
Verlag:
Springer Science and Business Media LLC
Publikationsdatum:
2021
ZDB Id:
3107320-7
ZDB Id:
2421351-2
