In:
Journal of Leukocyte Biology, Oxford University Press (OUP), Vol. 101, No. 4 ( 2017-04-01), p. 957-966
Kurzfassung:
Osteoclasts are cells specialized in bone resorption. Currently, studies on murine osteoclasts are primarily performed on bone marrow–derived cells with the use of many animals and limited cells available. ER-Hoxb8 cells are conditionally immortalized monocyte/macrophage murine progenitor cells, recently described to be able to differentiate toward functional osteoclasts. Here, we produced an ER-Hoxb8 clonal cell line from C57BL/6 bone marrow cells that strongly resembles phenotype and function of the conventional bone marrow–derived osteoclasts. We then used CRISPR/Cas9 technology to specifically inactivate genes by biallelic mutation. The CRISPR/Cas9 system is an adaptive immune system in Bacteria and Archaea and uses small RNAs and Cas nucleases to degrade foreign nucleic acids. Through specific-guide RNAs, the nuclease Cas9 can be redirected toward any genomic location to genetically modify eukaryotic cells. We genetically modified ER-Hoxb8 cells with success, generating NFATc1−/− and DC-STAMP−/− ER-Hoxb8 cells that lack the ability to differentiate into osteoclasts or to fuse into multinucleated osteoclasts, respectively. In conclusion, this method represents a markedly easy highly specific and efficient system for generating potentially unlimited numbers of genetically modified osteoclast precursors.
Materialart:
Online-Ressource
ISSN:
0741-5400
,
1938-3673
DOI:
10.1189/jlb.1AB0416-180RR
Sprache:
Englisch
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
2017
ZDB Id:
2026833-6
SSG:
12
