In:
Annals of the New York Academy of Sciences, Wiley, Vol. 1042, No. 1 ( 2005-05), p. 448-459
Abstract:
A bstract : 2,6‐Diisopropylphenol is an intravenous anesthetic agent used for induction and maintenance of anesthesia. Since it is similar to α‐tocopherol, 2,6‐diisopropylphenol may have antioxidant effects. Osteoblasts play important roles in bone remodeling. In this study, we attempted to evaluate the protective effects of 2,6‐diisopropylphenol on oxidative stress‐induced osteoblast insults and their possible mechanisms, using neonatal rat calvarial osteoblasts as the experimental model. Clinically relevant concentrations of 2,6‐diisopropylphenol (3 and 30 μM) had no effect on osteoblast viability. However, 2,6‐diisopropylphenol at 300 μM time‐dependently caused osteoblast death. Exposure to sodium nitroprusside (SNP), a nitric oxide donor, increased amounts of nitrite in osteoblasts. 2,6‐Diisopropylphenol did not scavenge basal or SNP‐releasing nitric oxide. Hydrogen peroxide (HP) enhanced levels of intracellular reactive oxygen species in osteoblasts. 2,6‐Diisopropylphenol significantly reduced HP‐induced oxidative stress. Exposure of osteoblasts to SNP and HP decreased cell viability time‐dependently. 2,6‐Diisopropylphenol protected osteoblasts from SNP‐ and HP‐induced cell damage. Analysis by a flow cytometric method revealed that SNP and HP induced osteoblast apoptosis. 2,6‐Diisopropylphenol significantly blocked SNP‐ and HP‐induced osteoblast apoptosis. Administration of SNP and HP increased caspase‐3 activities. However, 2,6‐diisopropylphenol significantly decreased SNP‐ and HP‐enhanced caspase‐3 activities. This study shows that a therapeutic concentration of 2,6‐diisopropylphenol can protect osteoblasts from SNP‐ and HP‐induced cell insults, possibly via suppression of caspase‐3 activities.
Type of Medium:
Online Resource
ISSN:
0077-8923
,
1749-6632
DOI:
10.1196/annals.1338.038
Language:
English
Publisher:
Wiley
Publication Date:
2005
detail.hit.zdb_id:
2834079-6
detail.hit.zdb_id:
211003-9
detail.hit.zdb_id:
2071584-5
SSG:
11
