In:
Annals of the New York Academy of Sciences, Wiley, Vol. 1090, No. 1 ( 2006-12), p. 188-202
Kurzfassung:
Abstract: Hepatocyte growth factor/scatter factor (HGF/SF) induces scattering, morphogenesis, and survival of epithelial cells through activation of the MET tyrosine kinase receptor. HGF/SF and MET are involved in normal development and tumor progression of many tissues and organs, including the mammary gland. In order to find target genes of HGF/SF involved in its survival function, we used an oligonucleotide microarray representing 1,920 genes known to be involved in apoptosis, transcriptional regulation, and signal transduction. MCF‐10A human mammary epithelial cells were grown in the absence of serum and treated or not with HGF/SF for 2 h. Total RNA was reverse‐transcribed to cDNA in the presence of fluorescent Cy3‐dUTP or Cy5‐dUTP to generate fluorescently labeled cDNA probes. Microarrays were performed and the ratios of Cy5/Cy3 fluorescence were determined. The expression of three apoptotic genes was modified by HGF/SF, with A20 being upregulated, and DAXX and SMAC being downregulated. These changes of expression were confirmed by real‐time quantitative PCR. According to current‐knowledge, A20 is antiapoptotic and SMAC is proapoptotic, while a pro‐ or antiapoptotic function of DAXX is controversial. The fact that HGF/SF upregulates an antiapoptotic gene (A20) and downregulates a proapoptotic gene (SMAC) is in agreement with its survival effect in MCF‐10A cells. This study identified novel apoptotic genes regulated by HGF/SF, which can contribute to its survival effect.
Materialart:
Online-Ressource
ISSN:
0077-8923
,
1749-6632
DOI:
10.1196/annals.1378.021
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2006
ZDB Id:
2834079-6
ZDB Id:
211003-9
ZDB Id:
2071584-5
SSG:
11
