In:
The Journal of Clinical Endocrinology & Metabolism, The Endocrine Society, Vol. 88, No. 4 ( 2003-04-01), p. 1809-1814
Abstract:
Human endometrium is infiltrated by natural killer (NK) cells throughout the menstrual cycle. The number of endometrial NK cells is low in the proliferative phase, but acutely increases after ovulation, and reaches a peak in the late secretory phase, suggesting that endometrium recruits these leukocytes selectively from circulating peripheral blood. We investigated the expression of macrophage inflammatory protein (MIP)-1β, a potential chemoattractant for NK cells, in the endometrium. RT-PCR and ELISA revealed that MIP-1β is expressed in the endometrium throughout the menstrual cycle at both the message and protein levels. MIP-1β expression is stronger in the secretory phase endometrium than in the proliferative phase endometrium. Immunohistochemistry revealed that MIP-1β is localized in the surface epithelial cells, glandular epithelial cells, and perivascular stromal cells throughout the menstrual cycle. Stromal cells in a wider perivascular area became immunoreactive in the secretory phase. There was a strong correlation between the endometrial MIP-1β concentration and the number of endometrial NK cells. Progesterone significantly induced MIP-1β secretion from cultured endometrial stromal cells, whereas 17β-estradiol had a weak effect. These results suggest that endometrial MIP-1β may be involved in the recruitment of NK cells from circulating peripheral blood.
Type of Medium:
Online Resource
ISSN:
0021-972X
,
1945-7197
DOI:
10.1210/jc.2002-020980
Language:
English
Publisher:
The Endocrine Society
Publication Date:
2003
detail.hit.zdb_id:
2026217-6
