In:
Biology Open, The Company of Biologists
Abstract:
Intraflagellar transport (IFT) particles are composed of polyprotein complexes IFT-A and IFT-B as well as cargo adaptors such as the BBSome. As two IFT-B subunits, IFT25 and IFT27 were found to form a heterodimer, which is essential in exporting the BBSome out of the cilium but not involved in flagellar assembly and cytokinesis in vertebrates. Controversial results were, however, recorded to show that defects in IFT, flagellar assembly and even cytokinesis were caused by IFT27 knockdown in C. reinhardtii. By using C. reinhardtii as a model organism, we report that, depletion of IFT25 has no effect on flagellar assembly and does not affect the entry of the BBSome into the flagellum but impaired its movement out of the flagellum, clarifying the evolutionally conserved role of IFT25 in regulating the exit of the BBSome from the flagellum cross species. Interestingly, depletion of IFT25 causes dramatic reduction of IFT27 as expected, which, however, does not cause defects in flagellar assembly and cytokinesis in C. reinhardtii. Our data thus support that Chlamydomonas IFT27, like its vertebrate homologues, is not involved in flagellar assembly and cytokinesis.
Type of Medium:
Online Resource
ISSN:
2046-6390
Language:
English
Publisher:
The Company of Biologists
Publication Date:
2017
detail.hit.zdb_id:
2632264-X
