In:
Development, The Company of Biologists, Vol. 111, No. 1 ( 1991-01-01), p. 233-244
Kurzfassung:
Carbonic anhydrase HI (CAHI) is an abundant soluble protein in adult mammalian slow twitch skeletal muscle fibers. It is thought to be an early marker for myogenesis based upon its high level of expression in myoblasts in vitro prior to fusion. Using in situ hybridization, we have studied the in vivo distribution of CAIH gene transcripts in mouse embryos and fetuses from 7.25 days to 17.5 days post coitum (p.c.). CAIH mRNAs are first detected in the myotomes of somites between 9.5 and 10.5 days p.c. (20–30 somites). At 15.5 daysp.c., CAIH begins to be restricted to developing slow muscle fibers. By two weeks post partum (p.p.), CAIH mRNAs are detected mainly in slow muscle fibers. CAHI transcripts are detected at an earlier stage (7.25 days p.c.) in the developing notochord. CAIH is expressed at a much higher level in the notochord than it is in developing skeletal muscle. As the notochord forms the nucleus pulposus in fetal mice, CAIH mRNA levels remain very high. Expression of CAIH in the notochord is of interest in the context of skeletal myogenesis because the notochord is thought to play an important role in somite formation. In addition to the notochord, CAIH transcripts are detected prenatally in several other non-muscle tissues: in cells of the choroid plexus, endocardial cushion and ureter, and in adipocytes.
Materialart:
Online-Ressource
ISSN:
0950-1991
,
1477-9129
DOI:
10.1242/dev.111.1.233
Sprache:
Englisch
Verlag:
The Company of Biologists
Publikationsdatum:
1991
ZDB Id:
2007916-3
SSG:
12
