In:
Development, The Company of Biologists
Kurzfassung:
Neuroendocrine cells in the pineal gland release melatonin during the night and in teleosts are directly photoreceptive. During development of the pineal complex, a small number of cells migrate leftward away from the pineal anlage to form the parapineal cell cluster, a process which is crucial for asymmetrical development of the bilateral habenular nuclei. Here we show that, throughout zebrafish embryonic development, the brain-specific homeobox (bsx) gene is expressed in all cell types of the pineal complex. We identified Bmp and Noto/Flh as major regulators of bsx expression in the pineal complex. Upon loss of Bsx through the generation of a targeted mutation, embryos fail to form a parapineal organ and develop right-isomerized habenulae. Crucial enzymes in the melatonin biosynthesis pathway are not expressed, suggesting absence of melatonin from the pineal gland of bsx mutants. Several genes involved in rod-like or cone-like phototransduction are also abnormally expressed, indicating that Bsx plays a pivotal role in differentiation of multiple cell types in the zebrafish pineal complex.
Materialart:
Online-Ressource
ISSN:
1477-9129
,
0950-1991
Sprache:
Englisch
Verlag:
The Company of Biologists
Publikationsdatum:
2018
ZDB Id:
2007916-3
SSG:
12
