In:
Development, The Company of Biologists
Kurzfassung:
The key molecular interactions governing vertebrate limb bud development are a paradigm to study the mechanisms controlling progenitor cell proliferation and specification during vertebrate organogenesis. However, little is known about the cellular heterogeneity of the mesenchymal progenitors in early limb buds that ultimately contribute to the chondrogenic condensations prefiguring the skeleton. We combined flow cytometric and transcriptome analyses to identify the molecular signatures of several distinct mesenchymal progenitor cell populations present in early mouse forelimb buds. In particular, JAGGED1 (JAG1)-positive cells located in the posterior-distal mesenchyme were identified as the most immature limb bud mesenchymal progenitors (LMPs), which critically depend on SHH and FGF signaling in culture. The analysis of Gremlin1 (Grem1)-deficient forelimb buds showed that JAG1-expressing LMPs are protected from apoptosis by GREM1-mediated BMP antagonism. At the same stage, the osteo-chondrogenic progenitors (OCPs) located in the core mesenchyme are already actively responding to BMP signaling. This analysis sheds light on the cellular heterogeneity of the early mouse limb bud mesenchyme and the distinct response of LMPs and OCPs to morphogen signaling.
Materialart:
Online-Ressource
ISSN:
1477-9129
,
0950-1991
Sprache:
Englisch
Verlag:
The Company of Biologists
Publikationsdatum:
2019
ZDB Id:
2007916-3
SSG:
12
