In:
Journal of Cell Science, The Company of Biologists
Kurzfassung:
Vinexin, CAP and ArgBP2 constitute an adaptor protein family called the vinexin (SORBS) family that is targeted to focal adhesions (FAs). Although numerous studies have focused on each of the SORBS proteins and partially elucidated their involvement in mechanotransduction, a comparative analysis of their function has not been well addressed. Here, we established mouse embryonic fibroblasts that individually expressed SORBS proteins and analyzed their functions in an identical cell context. Both vinexin α and CAP colocalized with vinculin at FAs and promoted the appearance of vinculin-rich FAs, whereas ArgBP2 colocalized with α-actinin at the proximal end of FAs and punctate structures on actin stress fibers (SFs), and induced paxillin-rich FAs. Furthermore, both vinexin α and CAP contributed to extracellular matrix stiffness-dependent vinculin behaviors, while ArgBP2 stabilized α-actinin on SFs and enhanced intracellular contractile forces. These results demonstrate the differential roles of SORBS proteins in mechanotransduction.
Materialart:
Online-Ressource
ISSN:
1477-9137
,
0021-9533
Sprache:
Englisch
Verlag:
The Company of Biologists
Publikationsdatum:
2017
ZDB Id:
219171-4
ZDB Id:
1483099-1
SSG:
12
