In:
Biomedical Optics Express, Optica Publishing Group, Vol. 13, No. 12 ( 2022-12-01), p. 6610-
Abstract:
A temporal focusing multiphoton illumination (TFMI) method is proposed for achieving selective volume illumination (SVI) (i.e., illuminating only the volume of interest) in light-field microscopy (LFM). The proposed method minimizes the background noise of the LFM images and enhances the contrast, and thus improves the imaging quality. Three-dimensional (3D) volumetric imaging is achieved by reconstructing the LFM images using a phase-space deconvolution algorithm. The experimental results obtained using 100-nm fluorescent beads show that the proposed TFMI-LFM system achieves lateral and axial resolutions of 1.2 µm and 1.1 µm, respectively, at the focal plane. Furthermore, the TFMI-LFM system enables 3D images of the single lobe of the drosophila mushroom body with GFP biomarker (OK-107) to be reconstructed in a one-snapshot record.
Type of Medium:
Online Resource
ISSN:
2156-7085
,
2156-7085
Language:
English
Publisher:
Optica Publishing Group
Publication Date:
2022
detail.hit.zdb_id:
2572216-5