In:
Journal of Histochemistry & Cytochemistry, SAGE Publications, Vol. 57, No. 12 ( 2009-12), p. 1127-1137
Abstract:
Somatostatin released from capsaicin-sensitive sensory nerves of the lung during endotoxin-induced murine pneumonitis inhibits inflammation and hyperresponsiveness, presumably via somatostatin receptor subtype 4 (sst 4 ). The goal of the present study was to identify sst 4 receptors in mouse and human lungs and to reveal its inflammation-induced alterations with real-time quantitative PCR, Western blot, and immunohistochemistry. In non-inflamed mouse and human lungs, mRNA expression and immunolocalization of sst 4 are very similar. They are present on bronchial epithelial, vascular endothelial, and smooth-muscle cells. The sst 4 receptor protein in the mouse lung significantly increases 24 hr after intranasal endotoxin administration as well as in response to 3 months of whole-body cigarette smoke exposure, owing to the infiltrating sst 4 -positivite mononuclear cells and neutrophils. In the chronically inflamed human lung, the large number of activated macrophages markedly elevate sst 4 mRNA levels, although there is no change in acute purulent pneumonia, in which granulocytes accumulate. Despite mouse granulocytes, human neutrophils do not show sst 4 immunopositivity. We provide the first evidence for the expression, localization, and inflammation-induced alterations of sst 4 receptors in murine and human lungs. Inasmuch as tissue distribution of this receptor is highly similar, extrapolation of murine experimental results to human conditions might be possible.
Type of Medium:
Online Resource
ISSN:
0022-1554
,
1551-5044
DOI:
10.1369/jhc.2009.953919
Language:
English
Publisher:
SAGE Publications
Publication Date:
2009
detail.hit.zdb_id:
1421306-0
SSG:
12