In:
PLOS Biology, Public Library of Science (PLoS), Vol. 19, No. 3 ( 2021-3-29), p. e3001158-
Kurzfassung:
Since its emergence in December 2019, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has spread globally and become a major public health burden. Despite its close phylogenetic relationship to SARS-CoV, SARS-CoV-2 exhibits increased human-to-human transmission dynamics, likely due to efficient early replication in the upper respiratory epithelium of infected individuals. Since different temperatures encountered in the human upper and lower respiratory tract (33°C and 37°C, respectively) have been shown to affect the replication kinetics of several respiratory viruses, as well as host innate immune response dynamics, we investigated the impact of temperature on SARS-CoV-2 and SARS-CoV infection using the primary human airway epithelial cell culture model. SARS-CoV-2, in contrast to SARS-CoV, replicated to higher titers when infections were performed at 33°C rather than 37°C. Although both viruses were highly sensitive to type I and type III interferon pretreatment, a detailed time-resolved transcriptome analysis revealed temperature-dependent interferon and pro-inflammatory responses induced by SARS-CoV-2 that were inversely proportional to its replication efficiency at 33°C or 37°C. These data provide crucial insight on pivotal virus–host interaction dynamics and are in line with characteristic clinical features of SARS-CoV-2 and SARS-CoV, as well as their respective transmission efficiencies.
Materialart:
Online-Ressource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001158
DOI:
10.1371/journal.pbio.3001158.g001
DOI:
10.1371/journal.pbio.3001158.g002
DOI:
10.1371/journal.pbio.3001158.g003
DOI:
10.1371/journal.pbio.3001158.g004
DOI:
10.1371/journal.pbio.3001158.s001
DOI:
10.1371/journal.pbio.3001158.s002
DOI:
10.1371/journal.pbio.3001158.s003
DOI:
10.1371/journal.pbio.3001158.s004
DOI:
10.1371/journal.pbio.3001158.s005
DOI:
10.1371/journal.pbio.3001158.s006
DOI:
10.1371/journal.pbio.3001158.s007
DOI:
10.1371/journal.pbio.3001158.s008
DOI:
10.1371/journal.pbio.3001158.s009
DOI:
10.1371/journal.pbio.3001158.s010
DOI:
10.1371/journal.pbio.3001158.s011
DOI:
10.1371/journal.pbio.3001158.s012
DOI:
10.1371/journal.pbio.3001158.s013
DOI:
10.1371/journal.pbio.3001158.s014
DOI:
10.1371/journal.pbio.3001158.r001
DOI:
10.1371/journal.pbio.3001158.r002
DOI:
10.1371/journal.pbio.3001158.r003
DOI:
10.1371/journal.pbio.3001158.r004
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2021
ZDB Id:
2126773-X
