In:
PLOS Biology, Public Library of Science (PLoS), Vol. 21, No. 3 ( 2023-3-22), p. e3001879-
Abstract:
Bacteria that live inside the cells of insect hosts (endosymbionts) can alter the reproduction of their hosts, including the killing of male offspring (male killing, MK). MK has only been described in a few insects, but this may reflect challenges in detecting MK rather than its rarity. Here, we identify MK Wolbachia at a low frequency (around 4%) in natural populations of Drosophila pseudotakahashii . MK Wolbachia had a stable density and maternal transmission during laboratory culture, but the MK phenotype which manifested mainly at the larval stage was lost rapidly. MK Wolbachia occurred alongside a second Wolbachia strain expressing a different reproductive manipulation, cytoplasmic incompatibility (CI). A genomic analysis highlighted Wolbachia regions diverged between the 2 strains involving 17 genes, and homologs of the wmk and cif genes implicated in MK and CI were identified in the Wolbachia assembly. Doubly infected males induced CI with uninfected females but not females singly infected with CI-causing Wolbachia . A rapidly spreading dominant nuclear suppressor genetic element affecting MK was identified through backcrossing and subsequent analysis with ddRAD SNPs of the D . pseudotakahashii genome. These findings highlight the complexity of nuclear and microbial components affecting MK endosymbiont detection and dynamics in populations and the challenges of making connections between endosymbionts and the host phenotypes affected by them.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001879
DOI:
10.1371/journal.pbio.3001879.g001
DOI:
10.1371/journal.pbio.3001879.g002
DOI:
10.1371/journal.pbio.3001879.g003
DOI:
10.1371/journal.pbio.3001879.t001
DOI:
10.1371/journal.pbio.3001879.t002
DOI:
10.1371/journal.pbio.3001879.t003
DOI:
10.1371/journal.pbio.3001879.t004
DOI:
10.1371/journal.pbio.3001879.t005
DOI:
10.1371/journal.pbio.3001879.t006
DOI:
10.1371/journal.pbio.3001879.s001
DOI:
10.1371/journal.pbio.3001879.s002
DOI:
10.1371/journal.pbio.3001879.s003
DOI:
10.1371/journal.pbio.3001879.s004
DOI:
10.1371/journal.pbio.3001879.s005
DOI:
10.1371/journal.pbio.3001879.s006
DOI:
10.1371/journal.pbio.3001879.s007
DOI:
10.1371/journal.pbio.3001879.s008
DOI:
10.1371/journal.pbio.3001879.s009
DOI:
10.1371/journal.pbio.3001879.s010
DOI:
10.1371/journal.pbio.3001879.s011
DOI:
10.1371/journal.pbio.3001879.s012
DOI:
10.1371/journal.pbio.3001879.r001
DOI:
10.1371/journal.pbio.3001879.r002
DOI:
10.1371/journal.pbio.3001879.r003
DOI:
10.1371/journal.pbio.3001879.r004
DOI:
10.1371/journal.pbio.3001879.r005
DOI:
10.1371/journal.pbio.3001879.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2126773-X