In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 18, No. 8 ( 2022-8-29), p. e1010349-
Abstract:
A network of transcription factors (TFs) coordinates transcription with cell cycle events in eukaryotes. Most TFs in the network are phosphorylated by cyclin-dependent kinase (CDK), which limits their activities during the cell cycle. Here, we investigate the physiological consequences of disrupting CDK regulation of the paralogous repressors Yhp1 and Yox1 in yeast. Blocking Yhp1/Yox1 phosphorylation increases their levels and decreases expression of essential cell cycle regulatory genes which, unexpectedly, increases cellular fitness in optimal growth conditions. Using synthetic genetic interaction screens, we find that Yhp1/Yox1 mutations improve the fitness of mutants with mitotic defects, including condensin mutants. Blocking Yhp1/Yox1 phosphorylation simultaneously accelerates the G1/S transition and delays mitotic exit, without decreasing proliferation rate. This mitotic delay partially reverses the chromosome segregation defect of condensin mutants, potentially explaining their increased fitness when combined with Yhp1/Yox1 phosphomutants. These findings reveal how altering expression of cell cycle genes leads to a redistribution of cell cycle timing and confers a fitness advantage to cells.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010349
DOI:
10.1371/journal.pgen.1010349.g001
DOI:
10.1371/journal.pgen.1010349.g002
DOI:
10.1371/journal.pgen.1010349.g003
DOI:
10.1371/journal.pgen.1010349.g004
DOI:
10.1371/journal.pgen.1010349.g005
DOI:
10.1371/journal.pgen.1010349.g006
DOI:
10.1371/journal.pgen.1010349.g007
DOI:
10.1371/journal.pgen.1010349.s001
DOI:
10.1371/journal.pgen.1010349.s002
DOI:
10.1371/journal.pgen.1010349.s003
DOI:
10.1371/journal.pgen.1010349.s004
DOI:
10.1371/journal.pgen.1010349.s005
DOI:
10.1371/journal.pgen.1010349.s006
DOI:
10.1371/journal.pgen.1010349.s007
DOI:
10.1371/journal.pgen.1010349.s008
DOI:
10.1371/journal.pgen.1010349.s009
DOI:
10.1371/journal.pgen.1010349.s010
DOI:
10.1371/journal.pgen.1010349.s011
DOI:
10.1371/journal.pgen.1010349.s012
DOI:
10.1371/journal.pgen.1010349.s013
DOI:
10.1371/journal.pgen.1010349.s014
DOI:
10.1371/journal.pgen.1010349.s015
DOI:
10.1371/journal.pgen.1010349.s016
DOI:
10.1371/journal.pgen.1010349.s017
DOI:
10.1371/journal.pgen.1010349.s018
DOI:
10.1371/journal.pgen.1010349.s019
DOI:
10.1371/journal.pgen.1010349.s020
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2186725-2