In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 16, No. 9 ( 2022-9-6), p. e0010740-
Abstract:
Invasive non-typhoidal Salmonella (iNTS–mainly serotypes Enteritidis and Typhimurium) are major causes of bloodstream infections in children in sub-Saharan Africa, but their reservoir remains unknown. We assessed iNTS carriage in rats in an urban setting endemic for iNTS carriage and compared genetic profiles of iNTS from rats with those isolated from humans. Methodology/Principal findings From April 2016 to December 2018, rats were trapped in five marketplaces and a slaughterhouse in Kisangani, Democratic Republic of the Congo. After euthanasia, blood, liver, spleen, and rectal content were cultured for Salmonella . Genetic relatedness between iNTS from rats and humans—obtained from blood cultures at Kisangani University Hospital—was assessed with multilocus variable-number tandem repeat (VNTR) analysis (MLVA), multilocus sequence typing (MLST) and core-genome MLST (cgMLST). 1650 live-capture traps yielded 566 (34.3%) rats (95.6% Rattus norvegicus , 4.4% Rattus rattus ); 46 (8.1%) of them carried Salmonella , of which 13 had more than one serotype. The most common serotypes were II.42:r:- (n = 18 rats), Kapemba (n = 12), Weltevreden and Typhimurium (n = 10, each), and Dublin (n = 8). Salmonella Typhimurium belonged to MLST ST19 (n = 7 rats) and the invasive ST313 (n = 3, isolated from deep organs but not from rectal content). Sixteen human S . Typhimurium isolates (all ST313) were available for comparison: MLVA and cgMLST revealed two distinct rat-human clusters involving both six human isolates, respectively, i . e . in total 12/16 human ST313 isolates. All ST313 Typhimurium isolates from rats and humans clustered with the ST313 Lineage 2 isolates and most were multidrug resistant; the remaining isolates from rats including S . Typhimurium ST19 were pan-susceptible. Conclusion The present study provides evidence of urban rats as potential reservoirs of S . Typhimurium ST313 in an iNTS endemic area in sub-Saharan Africa.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0010740
DOI:
10.1371/journal.pntd.0010740.g001
DOI:
10.1371/journal.pntd.0010740.g002
DOI:
10.1371/journal.pntd.0010740.g003
DOI:
10.1371/journal.pntd.0010740.g004
DOI:
10.1371/journal.pntd.0010740.g005
DOI:
10.1371/journal.pntd.0010740.t001
DOI:
10.1371/journal.pntd.0010740.t002
DOI:
10.1371/journal.pntd.0010740.t003
DOI:
10.1371/journal.pntd.0010740.t004
DOI:
10.1371/journal.pntd.0010740.s001
DOI:
10.1371/journal.pntd.0010740.s002
DOI:
10.1371/journal.pntd.0010740.s003
DOI:
10.1371/journal.pntd.0010740.s004
DOI:
10.1371/journal.pntd.0010740.s005
DOI:
10.1371/journal.pntd.0010740.s006
DOI:
10.1371/journal.pntd.0010740.r001
DOI:
10.1371/journal.pntd.0010740.r002
DOI:
10.1371/journal.pntd.0010740.r003
DOI:
10.1371/journal.pntd.0010740.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2429704-5