In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 17, No. 7 ( 2023-7-17), p. e0011481-
Abstract:
Iron is an essential element required for all organisms. Iron response regulator (Irr) is a crucial transcriptional regulator and can affect the growth and iron uptake of Brucella . The growth rate of Brucella melitensis M5-90 irr mutant was significantly lower than that of B . melitensis M5-90 under normal or iron-sufficient conditions, however, the growth rate of the B . melitensis M5-90 irr mutant was significantly higher than that of B . melitensis M5-90 under iron-limited conditions. In addition, irr mutation significantly reduced iron uptake under iron-limited conditions. Previous studies suggested that the Irr protein has multiple target genes in the Brucella genome that are involved in iron metabolism. Therefore, in the present study, a Dap-seq approach was used to investigate the other iron metabolism genes that are also regulated by the Irr protein in Brucella . A total of seven genes were identified as target genes for Irr in this study and the expression levels of these seven genes was identified using qRT-PCR. The electrophoretic mobility shift assay confirmed that six out of the seven genes, namely rirA (BME_RS13665), membrane protein (BME_RS01725), hypothetical protein (BME_RS09560), ftrA (BME_RS14525), cation-transporting P-type ATPase (zntA) (BME_RS10660), and 2Fe-2S binding protein (BME_RS13655), interact with the Irr protein. Furthermore, the iron utilization and growth assay experiments confirmed that rirA was involve in iron metabolism and growth of Brucella . In summary, our results identified six genes regulated by the Irr protein that may participate in iron metabolism, and the rirA was identified as a regulon of Irr and it also plays a role in iron metabolism of Brucella . Collectively, these results provide valuable insights for the exploration of Brucella iron metabolism.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0011481
DOI:
10.1371/journal.pntd.0011481.g001
DOI:
10.1371/journal.pntd.0011481.g002
DOI:
10.1371/journal.pntd.0011481.g003
DOI:
10.1371/journal.pntd.0011481.g004
DOI:
10.1371/journal.pntd.0011481.g005
DOI:
10.1371/journal.pntd.0011481.g006
DOI:
10.1371/journal.pntd.0011481.g007
DOI:
10.1371/journal.pntd.0011481.g008
DOI:
10.1371/journal.pntd.0011481.s001
DOI:
10.1371/journal.pntd.0011481.s002
DOI:
10.1371/journal.pntd.0011481.s003
DOI:
10.1371/journal.pntd.0011481.s004
DOI:
10.1371/journal.pntd.0011481.s005
DOI:
10.1371/journal.pntd.0011481.s006
DOI:
10.1371/journal.pntd.0011481.s007
DOI:
10.1371/journal.pntd.0011481.s008
DOI:
10.1371/journal.pntd.0011481.s009
DOI:
10.1371/journal.pntd.0011481.s010
DOI:
10.1371/journal.pntd.0011481.s011
DOI:
10.1371/journal.pntd.0011481.s012
DOI:
10.1371/journal.pntd.0011481.s013
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2429704-5
