In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 1 ( 2021-1-28), p. e0246033-
Abstract:
Many animal and plant pathogenic bacteria employ a type three secretion system (T3SS) to deliver type three effector proteins (T3Es) into host cells. Efficient secretion of many T3Es in the plant pathogen Xanthomonas campestris pv. campestris ( Xcc ) relies on the global chaperone HpaB. However, how the domain of HpaB itself affects effector translocation/secretion is poorly understood. Here, we used genetic and biochemical approaches to identify a novel domain at the C-terminal end of HpaB (amino acid residues 137–160) that contributes to virulence and hypersensitive response (HR). Both in vitro secretion assay and in planta translocation assay showed that the secretion and translocation of T3E proteins depend on the C-terminal region of HpaB. Deletion of the C-terminal region of HpaB did not affect binding to T3Es, self-association or interaction with T3SS components. However, the deletion of C-terminal region sharply reduced the mounts of free T3Es liberated from the complex of HpaB with the T3Es, a reaction catalyzed in an ATP-dependent manner by the T3SS-associated ATPase HrcN. Our findings demonstrate the C-terminal domain of HpaB contributes to disassembly of chaperone-effector complex and reveal a potential molecular mechanism underpinning the involvement of HpaB in secretion of T3Es in Xcc .
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0246033
DOI:
10.1371/journal.pone.0246033.g001
DOI:
10.1371/journal.pone.0246033.g002
DOI:
10.1371/journal.pone.0246033.g003
DOI:
10.1371/journal.pone.0246033.g004
DOI:
10.1371/journal.pone.0246033.g005
DOI:
10.1371/journal.pone.0246033.g006
DOI:
10.1371/journal.pone.0246033.g007
DOI:
10.1371/journal.pone.0246033.g008
DOI:
10.1371/journal.pone.0246033.g009
DOI:
10.1371/journal.pone.0246033.s001
DOI:
10.1371/journal.pone.0246033.s002
DOI:
10.1371/journal.pone.0246033.s003
DOI:
10.1371/journal.pone.0246033.s004
DOI:
10.1371/journal.pone.0246033.s005
DOI:
10.1371/journal.pone.0246033.s006
DOI:
10.1371/journal.pone.0246033.s007
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3