In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 3 ( 2021-3-25), p. e0248985-
Kurzfassung:
There are limited treatments for dyschromia in burn hypertrophic scars (HTSs). Initial work in Duroc pig models showed that regions of scar that are light or dark have equal numbers of melanocytes. This study aims to confirm melanocyte presence in regions of hypo- and hyper-pigmentation in an animal model and patient samples. In a Duroc pig model, melanocyte presence was confirmed using en face staining. Patients with dyschromic HTSs had demographic, injury details, and melanin indices collected. Punch biopsies were taken of regions of hyper-, hypo-, or normally pigmented scar and skin. Biopsies were processed to obtain epidermal sheets (ESs). A subset of ESs were en face stained with melanocyte marker, S100β. Melanocytes were isolated from a different subset. Melanocytes were treated with NDP α-MSH, a pigmentation stimulator. mRNA was isolated from cells, and was used to evaluate gene expression of melanin-synthetic genes. In patient and pig scars, regions of hyper-, hypo-, and normal pigmentation had significantly different melanin indices. S100β en face staining showed that regions of hyper- and hypo-pigmentation contained the same number of melanocytes, but these cells had different dendricity/activity. Treatment of hypo-pigmented melanocytes with NDP α-MSH produced melanin by microscopy. Melanin-synthetic genes were upregulated in treated cells over controls. While traditionally it may be thought that hypopigmented regions of burn HTS display this phenotype because of the absence of pigment-producing cells, these data show that inactive melanocytes are present in these scar regions. By treating with a pigment stimulator, cells can be induced to re-pigment.
Materialart:
Online-Ressource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0248985
DOI:
10.1371/journal.pone.0248985.g001
DOI:
10.1371/journal.pone.0248985.g002
DOI:
10.1371/journal.pone.0248985.g003
DOI:
10.1371/journal.pone.0248985.g004
DOI:
10.1371/journal.pone.0248985.g005
DOI:
10.1371/journal.pone.0248985.g006
DOI:
10.1371/journal.pone.0248985.g007
DOI:
10.1371/journal.pone.0248985.g008
DOI:
10.1371/journal.pone.0248985.g009
DOI:
10.1371/journal.pone.0248985.g010
DOI:
10.1371/journal.pone.0248985.g011
DOI:
10.1371/journal.pone.0248985.g012
DOI:
10.1371/journal.pone.0248985.g013
DOI:
10.1371/journal.pone.0248985.g014
DOI:
10.1371/journal.pone.0248985.t001
DOI:
10.1371/journal.pone.0248985.t002
DOI:
10.1371/journal.pone.0248985.s001
DOI:
10.1371/journal.pone.0248985.s002
DOI:
10.1371/journal.pone.0248985.s003
DOI:
10.1371/journal.pone.0248985.s004
DOI:
10.1371/journal.pone.0248985.s005
DOI:
10.1371/journal.pone.0248985.s006
DOI:
10.1371/journal.pone.0248985.s007
DOI:
10.1371/journal.pone.0248985.s008
DOI:
10.1371/journal.pone.0248985.s009
DOI:
10.1371/journal.pone.0248985.s010
DOI:
10.1371/journal.pone.0248985.s011
DOI:
10.1371/journal.pone.0248985.s012
DOI:
10.1371/journal.pone.0248985.s013
DOI:
10.1371/journal.pone.0248985.s014
DOI:
10.1371/journal.pone.0248985.s015
DOI:
10.1371/journal.pone.0248985.s016
DOI:
10.1371/journal.pone.0248985.s017
DOI:
10.1371/journal.pone.0248985.r001
DOI:
10.1371/journal.pone.0248985.r002
DOI:
10.1371/journal.pone.0248985.r003
DOI:
10.1371/journal.pone.0248985.r004
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2021
ZDB Id:
2267670-3