In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 3 ( 2021-3-30), p. e0249341-
Abstract:
Bovine tuberculosis (bTB) caused by Mycobacterium bovis ( M . bovis ) represents one of major zoonotic diseases among cattle, it also affects the health of human, other domestic animals and wild life populations. Inhalation of infected aerosol droplets is considered as the most frequent route of the infection. This study aims to investigate the current forms of tuberculosis in cattle and identify the possible transmission modes in dairy farms of China. 13,345 cows from eight dairy farms in three provinces were comprehensively diagnosed by a multitude of assays, including SIT, CIT, IFN-γ assay and ELISA. It has been indicated that advanced infection of bTB was found in 752 (5.64%) cattle, suggesting a high prevalence of tuberculosis in these dairy farms. In the necropsy examination of 151 positive cattle, typical bTB lesions were observed in 131 cattle (86.75%), of which, notably, 90.84% lesions appeared in liver, spleen, mesenteric lymph nodes, mammary lymph nodes and other organs, taking up a large proportion among cattle with advanced bTB infection. 71.26% extrapulmonary tuberculosis (EPTB) was related to gastrointestinal system. M . bovis nucleic acid was further found in milk and feces samples and M . bovis was even isolated from milk samples. Phylogenetic analysis based on whole genome sequencing unraveled that six isolates were closely related to M . bovis AF2122/97 originated from UK, whereas four isolates shared close relation to M . bovis 30 from China, respectively. Our data demonstrate that the increase of EPTB transmitted by digestive tract is implicated in the current high prevalence rate of bTB in China, which also provides leads for bTB control in other countries with high prevalence of bTB in the future.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0249341
DOI:
10.1371/journal.pone.0249341.g001
DOI:
10.1371/journal.pone.0249341.g002
DOI:
10.1371/journal.pone.0249341.g003
DOI:
10.1371/journal.pone.0249341.g004
DOI:
10.1371/journal.pone.0249341.t001
DOI:
10.1371/journal.pone.0249341.t002
DOI:
10.1371/journal.pone.0249341.s001
DOI:
10.1371/journal.pone.0249341.s002
DOI:
10.1371/journal.pone.0249341.s003
DOI:
10.1371/journal.pone.0249341.s004
DOI:
10.1371/journal.pone.0249341.s005
DOI:
10.1371/journal.pone.0249341.s006
DOI:
10.1371/journal.pone.0249341.s007
DOI:
10.1371/journal.pone.0249341.r001
DOI:
10.1371/journal.pone.0249341.r002
DOI:
10.1371/journal.pone.0249341.r003
DOI:
10.1371/journal.pone.0249341.r004
DOI:
10.1371/journal.pone.0249341.r005
DOI:
10.1371/journal.pone.0249341.r006
DOI:
10.1371/journal.pone.0249341.r007
DOI:
10.1371/journal.pone.0249341.r008
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
