In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 4 ( 2021-4-30), p. e0250944-
Abstract:
Stroke is a common complication in children with tuberculous meningitis (TBM). Host proteins may give us insight into the mechanisms of stroke in TBM and serve as biomarkers for detection of stroke, however, they have not been widely explored. In this study, we compared the concentrations of cerebrospinal fluid (CSF) and serum proteins between children who had TBM-related stroke and children with TBM without stroke. Methods We collected CSF and serum from 47 children consecutively admitted to the Tygerberg Academic Hospital in Cape Town, South Africa between November 2016, and November 2017, on suspicion of having TBM. A multiplex platform was used to measure the concentrations of 69 host proteins in CSF and serum from all study participants. Results After classification of study participants, 23 (48.9%) out of the 47 study participants were diagnosed with TBM, of which 14 (60.9%) demonstrated radiological arterial ischemic infarction. The levels of lipocalin-2, sRAGE, IP-10/ CXCL10, sVCAM-1, MMP-1, and PDGF-AA in CSF samples and the levels of D-dimer, ADAMTS13, SAA, ferritin, MCP-1/ CCL2, GDF-15 and IL-13 in serum samples were statistically different between children who had TBM-related stroke and children with TBM without stroke. After correcting for multiple testing, only the levels of sVCAM-1, MMP-1, sRAGE, and IP-10/ CXCL10 in CSF were statistically different between the two groups. CSF and serum protein biosignatures indicated stroke in children diagnosed with TBM with up to 100% sensitivity and 88.9% specificity. Conclusion Serum and CSF proteins may serve as biomarkers for identifying individuals with stroke amongst children diagnosed with TBM at admission and may guide us to understand the biology of stroke in TBM. This was a pilot study, and thus further investigations in larger studies are needed.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0250944
DOI:
10.1371/journal.pone.0250944.g001
DOI:
10.1371/journal.pone.0250944.g002
DOI:
10.1371/journal.pone.0250944.g003
DOI:
10.1371/journal.pone.0250944.g004
DOI:
10.1371/journal.pone.0250944.g005
DOI:
10.1371/journal.pone.0250944.g006
DOI:
10.1371/journal.pone.0250944.g007
DOI:
10.1371/journal.pone.0250944.t001
DOI:
10.1371/journal.pone.0250944.t002
DOI:
10.1371/journal.pone.0250944.t003
DOI:
10.1371/journal.pone.0250944.s001
DOI:
10.1371/journal.pone.0250944.s002
DOI:
10.1371/journal.pone.0250944.s003
DOI:
10.1371/journal.pone.0250944.s004
DOI:
10.1371/journal.pone.0250944.s005
DOI:
10.1371/journal.pone.0250944.r001
DOI:
10.1371/journal.pone.0250944.r002
DOI:
10.1371/journal.pone.0250944.r003
DOI:
10.1371/journal.pone.0250944.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
