In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 2 ( 2022-2-17), p. e0254304-
Abstract:
MicroRNAs (miRNAs) are promising biomarkers in cancer research. Quantitative PCR (qPCR), also known as real-time PCR, is the most frequently used technique for measuring miRNA expression levels. The use of this technique, however, requires that expression data be normalized against reference genes. The problem is that a universal internal control for quantitative analysis of miRNA expression by qPCR has yet to be known. The aim of this work was to find the miRNAs with stable expression in the thyroid gland, brain and bone marrow according to NanoString nCounter miRNA quantification data. As a results, the most stably expressed miRNAs were as follows: miR-361-3p, -151a-3p and -29b-3p in the thyroid gland; miR-15a-5p, -194-5p and -532-5p in the brain; miR-140-5p, -148b-3p and -362-5p in bone marrow; and miR-423-5p, -28-5p and -532-5p, no matter what tissue type. These miRNAs represent promising reference genes for miRNA quantification by qPCR.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0254304
DOI:
10.1371/journal.pone.0254304.t001
DOI:
10.1371/journal.pone.0254304.t002
DOI:
10.1371/journal.pone.0254304.s001
DOI:
10.1371/journal.pone.0254304.s002
DOI:
10.1371/journal.pone.0254304.s003
DOI:
10.1371/journal.pone.0254304.s004
DOI:
10.1371/journal.pone.0254304.s005
DOI:
10.1371/journal.pone.0254304.s006
DOI:
10.1371/journal.pone.0254304.s007
DOI:
10.1371/journal.pone.0254304.s008
DOI:
10.1371/journal.pone.0254304.s009
DOI:
10.1371/journal.pone.0254304.r001
DOI:
10.1371/journal.pone.0254304.r002
DOI:
10.1371/journal.pone.0254304.r003
DOI:
10.1371/journal.pone.0254304.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3