In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 8 ( 2021-8-10), p. e0255075-
Kurzfassung:
Induced endothelial cells (iECs) generated from neonatal fibroblasts via transdifferentiation have been shown to have pro-angiogenic properties and are a potential therapy for peripheral arterial disease (PAD). It is unknown if iECs can be generated from fibroblasts collected from PAD patients and whether these cells are pro-angiogenic. In this study fibroblasts were collected from four PAD patients undergoing carotid endarterectomies. These cells, and neonatal fibroblasts, were transdifferentiated into iECs using modified mRNA. Endothelial phenotype and pro-angiogenic cytokine secretion were investigated. NOD-SCID mice underwent surgery to induce hindlimb ischaemia in a murine model of PAD. Mice received intramuscular injections with either control vehicle, or 1 × 10 6 neonatal-derived or 1 × 10 6 patient-derived iECs. Recovery in perfusion to the affected limb was measured using laser Doppler scanning. Perfusion recovery was enhanced in mice treated with neonatal-derived iECs and in two of the three patient-derived iEC lines investigated in vivo . Patient-derived iECs can be successfully generated from PAD patients and for specific patients display comparable pro-angiogenic properties to neonatal-derived iECs.
Materialart:
Online-Ressource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0255075
DOI:
10.1371/journal.pone.0255075.g001
DOI:
10.1371/journal.pone.0255075.g002
DOI:
10.1371/journal.pone.0255075.g003
DOI:
10.1371/journal.pone.0255075.g004
DOI:
10.1371/journal.pone.0255075.g005
DOI:
10.1371/journal.pone.0255075.t001
DOI:
10.1371/journal.pone.0255075.s001
DOI:
10.1371/journal.pone.0255075.s002
DOI:
10.1371/journal.pone.0255075.s003
DOI:
10.1371/journal.pone.0255075.s004
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2021
ZDB Id:
2267670-3