In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 7 ( 2021-7-23), p. e0255120-
Abstract:
The potential risks of environmental nanoparticles (NPs), in particular Polystyrene Nanoparticles (PNPs), is an emerging problem; specifically, the interaction of PNPs with intestinal cells has not been characterized so far. The mechanism by which polystyrene particles are transferred to humans has not yet been clarified, whether directly through ingestion from contaminated food. We evaluated the interaction between PNPs and colorectal adenocarcinoma cells (HCT116). Cells were exposed to different concentrations of PNPs, metabolic activity and the consequent cytotoxic potential were assessed through viability test; we evaluated the PNP genotoxic potential through the Cytokinesis-Block Micronucleus cytome (CBMN cyt) assay. Finally, we detected Reactive Oxygen Species (ROS) production after NPs exposure and performed Western Blot analysis to analyze the enzymes (SOD1, SOD2, Catalase, Glutathione Peroxidase) involved in the cell detoxification process that comes into play during the cell-PNPs interaction. This work analyzes the cyto and genotoxicity of PNPs in the colorectal HCT116 cell line, in particular the potential damage from oxidative stress produced by PNPs inside the cells related to the consequent nuclear damage. Our results show moderate toxicity of PNPs both in terms of ROS production and DNA damage. Further studies will be needed on different cell lines to have a more complete picture of the impact of environmental pollution on human health in terms of PNPs cytotoxicity and genotoxicity.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0255120
DOI:
10.1371/journal.pone.0255120.g001
DOI:
10.1371/journal.pone.0255120.g002
DOI:
10.1371/journal.pone.0255120.g003
DOI:
10.1371/journal.pone.0255120.g004
DOI:
10.1371/journal.pone.0255120.g005
DOI:
10.1371/journal.pone.0255120.g006
DOI:
10.1371/journal.pone.0255120.g007
DOI:
10.1371/journal.pone.0255120.g008
DOI:
10.1371/journal.pone.0255120.s001
DOI:
10.1371/journal.pone.0255120.s002
DOI:
10.1371/journal.pone.0255120.s003
DOI:
10.1371/journal.pone.0255120.s004
DOI:
10.1371/journal.pone.0255120.s005
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3