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LuNER: Multiplexed SARS-CoV-2 detection in clinical swab and wastewater samples

Stahl, Elizabeth C. ; Gopez, Allan R. ; Tsuchida, Connor A. ; [weitere]

Public Library of Science (PLoS) ; 2021

In: PLOS ONE Vol. 16, No. 11 ( 2021-11-10), p. e0258263-

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In: PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 11 ( 2021-11-10), p. e0258263-

Kurzfassung: Clinical and surveillance testing for the SARS-CoV-2 virus relies overwhelmingly on RT-qPCR-based diagnostics, yet several popular assays require 2–3 separate reactions or rely on detection of a single viral target, which adds significant time, cost, and risk of false-negative results. Furthermore, multiplexed RT-qPCR tests that detect at least two SARS-CoV-2 genes in a single reaction are typically not affordable for large scale clinical surveillance or adaptable to multiple PCR machines and plate layouts. We developed a RT-qPCR assay using the Luna Probe Universal One-Step RT-qPCR master mix with publicly available primers and probes to detect SARS-CoV-2 N gene, E gene, and human RNase P (LuNER) to address these shortcomings and meet the testing demands of a university campus and the local community. This cost-effective test is compatible with BioRad or Applied Biosystems qPCR machines, in 96 and 384-well formats, with or without sample pooling, and has a detection sensitivity suitable for both clinical reporting and wastewater surveillance efforts.

Materialart: Online-Ressource

ISSN: 1932-6203

URL: Article