In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 1 ( 2022-1-7), p. e0262416-
Abstract:
Mucosal infiltration by certain bacterial species may contribute to the development and progression of colorectal cancer (CRC). There is considerable variation in reported detection rates in human CRC samples and the extent to which bacterial infiltration varies across regions of the primary tumour is unknown. This study aimed to determine if there is an optimal site for bacterial detection within CRC tumours. Methods Presence of target bacterial species was assessed by quantitative real-time PCR (qPCR) in 42 human CRC tumours. Abundance in primary tumour regions, normal epithelium and at metastatic sites was investigated in an expanded cohort of 51 patients. Species presence/absence was confirmed by diversity profiling in five patients. Correlation with total bacterial load and clinicopathological features was assessed. Results Fusobacterium nucleatum and Bacteroides fragilis were detected in tumours from 43% and 24% of patients, respectively (17% positive for both species). The optimal detection site was the tumour luminal surface (TLS). Patients testing positive at the TLS frequently tested negative at other sites, including central tumour and invasive margin. F . nucleatum was detected at a higher frequency in tumour versus normal epithelium (p 〈 0.01) and was associated with more advanced disease (p = 0.01). Detection of both species correlated with total bacterial load. However, corroboration of qPCR results via diversity profiling suggests detection of these species may indicate a specific microbial signature. Conclusions This study supports a role for F . nucleatum in CRC development. Presence of F . nucleatum and B . fragilis varies across primary tumour regions, with the TLS representing the optimal site for bacterial detection.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0262416
DOI:
10.1371/journal.pone.0262416.g001
DOI:
10.1371/journal.pone.0262416.g002
DOI:
10.1371/journal.pone.0262416.g003
DOI:
10.1371/journal.pone.0262416.g004
DOI:
10.1371/journal.pone.0262416.t001
DOI:
10.1371/journal.pone.0262416.t002
DOI:
10.1371/journal.pone.0262416.s001
DOI:
10.1371/journal.pone.0262416.s002
DOI:
10.1371/journal.pone.0262416.s003
DOI:
10.1371/journal.pone.0262416.s004
DOI:
10.1371/journal.pone.0262416.s005
DOI:
10.1371/journal.pone.0262416.s006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3