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Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma

Power, Erica A. ; Fernandez-Torres, Jenelys ; Zhang, Liang ; [et al.]

Public Library of Science (PLoS) ; 2022

In: PLOS ONE Vol. 17, No. 2 ( 2022-2-14), p. e0263822-

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In: PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 2 ( 2022-2-14), p. e0263822-

Abstract: Diffuse intrinsic pontine glioma (DIPG) is a lethal pediatric brain tumor. While there are a number of in vivo rodent models for evaluating tumor biology and response to therapy, these models require significant time and resources. Here, we established the chick-embryo chorioallantoic (CAM) assay as an affordable and time efficient xenograft model for testing a variety of treatment approaches for DIPG. We found that patient-derived DIPG tumors develop in the CAM and maintain the same genetic and epigenetic characteristics of native DIPG tumors. We monitored tumor response to pharmaco- and radiation therapy by 3-D ultrasound volumetric and vasculature analysis. In this study, we established and validated the CAM model as a potential intermediate xenograft model for DIPG and its use for testing novel treatment approaches that include pharmacotherapy or radiation.

Type of Medium: Online Resource

ISSN: 1932-6203

URL: Article