In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 8 ( 2022-8-26), p. e0273802-
Kurzfassung:
Nervous Necrosis Virus (NNV) represents one of the most threatening pathogens for Mediterranean aquaculture. Several NNV strains are currently co-circulating in the Mediterranean Basin with a high prevalence of the RGNNV genotype and the RGNNV/SJNNV reassortant strain and a more limited diffusion of the SJNNV genotype and the SJNNV/RGNNV reassortant. In the present study, a one-step multiplex RT-PCR (mRT-PCR) assay was developed as an easy, cost-effective and rapid diagnostic technique to detect RGNNV and the reassortant RGNNV/SJNNV strain and to distinguish them from SJNNV and the reassortant SJNNV/RGNNV strain in a single RT-PCR reaction. A unique amplification profile was obtained for each genotype/reassortant enabling their rapid identification from cell culture lysates or directly from brain tissues of suspected fish. The method’s detection limit varied between 10 2.3 and 10 3.4 TCID 50 ml -1 depending on viral strains. No cross-reacitivty with viruses and bacteria frequently associated with gilthead seabream, European seabass and marine environment was observed. The mRT-PCR was shown to be an accurate, rapid and affordable method to support traditional diagnostic techniques in the diagnosis of VNN, being able to reduce considerably the time to identify the viral genotype or the involvement of reassortant strains.
Materialart:
Online-Ressource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0273802
DOI:
10.1371/journal.pone.0273802.g001
DOI:
10.1371/journal.pone.0273802.g002
DOI:
10.1371/journal.pone.0273802.g003
DOI:
10.1371/journal.pone.0273802.g004
DOI:
10.1371/journal.pone.0273802.g005
DOI:
10.1371/journal.pone.0273802.g006
DOI:
10.1371/journal.pone.0273802.t001
DOI:
10.1371/journal.pone.0273802.t002
DOI:
10.1371/journal.pone.0273802.s001
DOI:
10.1371/journal.pone.0273802.s002
DOI:
10.1371/journal.pone.0273802.s003
DOI:
10.1371/journal.pone.0273802.s004
DOI:
10.1371/journal.pone.0273802.s005
DOI:
10.1371/journal.pone.0273802.s006
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2022
ZDB Id:
2267670-3
