In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 1 ( 2021-1-13), p. e1009215-
Abstract:
Poxvirus systems have been extensively used as vaccine vectors. Herein a RNA-Seq analysis of intramuscular injection sites provided detailed insights into host innate immune responses, as well as expression of vector and recombinant immunogen genes, after vaccination with a new multiplication defective, vaccinia-based vector, Sementis Copenhagen Vector. Chikungunya and Zika virus immunogen mRNA and protein expression was associated with necrosing skeletal muscle cells surrounded by mixed cellular infiltrates. The multiple adjuvant signatures at 12 hours post-vaccination were dominated by TLR3, 4 and 9, STING, MAVS, PKR and the inflammasome. Th1 cytokine signatures were dominated by IFNγ, TNF and IL1β, and chemokine signatures by CCL5 and CXCL12. Multiple signatures associated with dendritic cell stimulation were evident. By day seven, vaccine transcripts were absent, and cell death, neutrophil, macrophage and inflammation annotations had abated. No compelling arthritis signatures were identified. Such injection site vaccinology approaches should inform refinements in poxvirus-based vector design.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009215
DOI:
10.1371/journal.ppat.1009215.g001
DOI:
10.1371/journal.ppat.1009215.g002
DOI:
10.1371/journal.ppat.1009215.g003
DOI:
10.1371/journal.ppat.1009215.g004
DOI:
10.1371/journal.ppat.1009215.g005
DOI:
10.1371/journal.ppat.1009215.g006
DOI:
10.1371/journal.ppat.1009215.s001
DOI:
10.1371/journal.ppat.1009215.s002
DOI:
10.1371/journal.ppat.1009215.s003
DOI:
10.1371/journal.ppat.1009215.s004
DOI:
10.1371/journal.ppat.1009215.s005
DOI:
10.1371/journal.ppat.1009215.s006
DOI:
10.1371/journal.ppat.1009215.s007
DOI:
10.1371/journal.ppat.1009215.s008
DOI:
10.1371/journal.ppat.1009215.s009
DOI:
10.1371/journal.ppat.1009215.r001
DOI:
10.1371/journal.ppat.1009215.r002
DOI:
10.1371/journal.ppat.1009215.r003
DOI:
10.1371/journal.ppat.1009215.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
