In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 10 ( 2021-10-21), p. e1009704-
Abstract:
Development of effective therapeutics for mitigating the COVID-19 pandemic is a pressing global need. Neutralizing antibodies are known to be effective antivirals, as they can be rapidly deployed to prevent disease progression and can accelerate patient recovery without the need for fully developed host immunity. Here, we report the generation and characterization of a series of chimeric antibodies against the receptor-binding domain (RBD) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein. Some of these antibodies exhibit exceptionally potent neutralization activities in vitro and in vivo , and the most potent of our antibodies target three distinct non-overlapping epitopes within the RBD. Cryo-electron microscopy analyses of two highly potent antibodies in complex with the SARS-CoV-2 spike protein suggested they may be particularly useful when combined in a cocktail therapy. The efficacy of this antibody cocktail was confirmed in SARS-CoV-2-infected mouse and hamster models as prophylactic and post-infection treatments. With the emergence of more contagious variants of SARS-CoV-2, cocktail antibody therapies hold great promise to control disease and prevent drug resistance.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009704
DOI:
10.1371/journal.ppat.1009704.g001
DOI:
10.1371/journal.ppat.1009704.g002
DOI:
10.1371/journal.ppat.1009704.g003
DOI:
10.1371/journal.ppat.1009704.g004
DOI:
10.1371/journal.ppat.1009704.g005
DOI:
10.1371/journal.ppat.1009704.g006
DOI:
10.1371/journal.ppat.1009704.g007
DOI:
10.1371/journal.ppat.1009704.g008
DOI:
10.1371/journal.ppat.1009704.s001
DOI:
10.1371/journal.ppat.1009704.s002
DOI:
10.1371/journal.ppat.1009704.s003
DOI:
10.1371/journal.ppat.1009704.s004
DOI:
10.1371/journal.ppat.1009704.s005
DOI:
10.1371/journal.ppat.1009704.s006
DOI:
10.1371/journal.ppat.1009704.s007
DOI:
10.1371/journal.ppat.1009704.s008
DOI:
10.1371/journal.ppat.1009704.s009
DOI:
10.1371/journal.ppat.1009704.s010
DOI:
10.1371/journal.ppat.1009704.s011
DOI:
10.1371/journal.ppat.1009704.s012
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
