In:
PLOS Pathogens, Public Library of Science (PLoS), Vol. 17, No. 8 ( 2021-8-16), p. e1009824-
Abstract:
The herpes simplex virus (HSV)-1 protein pUL21 is essential for efficient virus replication and dissemination. While pUL21 has been shown to promote multiple steps of virus assembly and spread, the molecular basis of its function remained unclear. Here we identify that pUL21 is a virus-encoded adaptor of protein phosphatase 1 (PP1). pUL21 directs the dephosphorylation of cellular and virus proteins, including components of the viral nuclear egress complex, and we define a conserved non-canonical linear motif in pUL21 that is essential for PP1 recruitment. In vitro evolution experiments reveal that pUL21 antagonises the activity of the virus-encoded kinase pUS3, with growth and spread of pUL21 PP1-binding mutant viruses being restored in adapted strains where pUS3 activity is disrupted. This study shows that virus-directed phosphatase activity is essential for efficient herpesvirus assembly and spread, highlighting the fine balance between kinase and phosphatase activity required for optimal virus replication.
Type of Medium:
Online Resource
ISSN:
1553-7374
DOI:
10.1371/journal.ppat.1009824
DOI:
10.1371/journal.ppat.1009824.g001
DOI:
10.1371/journal.ppat.1009824.g002
DOI:
10.1371/journal.ppat.1009824.g003
DOI:
10.1371/journal.ppat.1009824.g004
DOI:
10.1371/journal.ppat.1009824.g005
DOI:
10.1371/journal.ppat.1009824.g006
DOI:
10.1371/journal.ppat.1009824.s001
DOI:
10.1371/journal.ppat.1009824.s002
DOI:
10.1371/journal.ppat.1009824.s003
DOI:
10.1371/journal.ppat.1009824.s004
DOI:
10.1371/journal.ppat.1009824.s005
DOI:
10.1371/journal.ppat.1009824.s006
DOI:
10.1371/journal.ppat.1009824.s007
DOI:
10.1371/journal.ppat.1009824.s008
DOI:
10.1371/journal.ppat.1009824.s009
DOI:
10.1371/journal.ppat.1009824.r001
DOI:
10.1371/journal.ppat.1009824.r002
DOI:
10.1371/journal.ppat.1009824.r003
DOI:
10.1371/journal.ppat.1009824.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2205412-1
